* Andrew Garfield as Peter Parker / Spider-Man: An academic student who is bitten by a radioactive spider and acquires the agility and proportionate strength of an arachnid. He then uses this power for good and becomes a superhero.
* Emma Stone as Gwen Stacy: One of Spider-Man's earliest love interests in the comics.
* Rhys Ifans as Dr. Curt Connors / The Lizard: A scientist who tests lizard serum on himself that regenerates his hand, but also turns him into a reptilian humanoid monster.
* Martin Sheen as Ben Parker: May Parker's husband and Peter Parker's uncle.
* Sally Field as May Parker: Ben Parker's wife and Peter Parker's aunt.
* Denis Leary as Captain George Stacy: Gwen's father and a New York City Police Department captain.
* Chris Zylka as Eugene "Flash" Thompson: Peter Parker's high school nemesis, a football player who constantly bullies Peter dubbing him "Puny Parker" while also being a big fan of Spider-Man.
* Irrfan Khan as Van Adder: One of the villains in the movie. In the comics he is known to turn into the supervillain known as the Proto-Goblin.
* Annie Parisse as Van Adder's wife.
* Campbell Scott as Richard Parker: The father of Peter Parker.
* Julianne Nicholson as Mary Parker: The mother of Peter Parker.
* C. Thomas Howell as Ray.
* Miles Elliot as Billy Connors: The son of Dr. Curt Connors.
Spider-Man co-creator Stan Lee has a cameo in the next Spider-Man film, as he did in the previous Spider-Man films, which will involve him in an action scene and which he referred to as his "best, most unusual one yet!"
Released July 3, 2012